《植物生理学报》 2015, 51(11): 1855-1860

海洋生境芽孢杆菌(Bacillus sp.) T28菌株对番茄生长的多功能促进作用

崔荣强^1,2, 张久明¹, 马湘君¹, 李闯¹, 田黎^1,2,*

¹青岛科技大学生物系, 山东青岛266042; ²国家海洋局第一海洋研究所, 山东青岛266061

通信作者：田黎；E-mail: tianli@fio.org.cn；Tel: 0532-88967423

摘　要：

以来自海洋生境具有强抑菌作用的芽孢杆菌T28为试验菌株, 采用生物学与分子生物学方法研究其对番茄生长的多种促进作用。结果表明, T28菌株具有固氮酶基因nifH和固氮作用, 可以分解磷和钾, 产生铁载体和促生长植物激素IAA。标记的T28菌株可在番茄植株上定殖, 从而提高番茄苗的叶绿素含量, 定殖15 d后比对照的叶绿素含量高33.3%, 且差异显著。从T28菌株中扩增到γ-谷氨酰胺激酶(GK)基因proB, 推测T28菌株可能通过对脯氨酸的调节来诱导植物抗盐。病害防治试验证明, T28菌株对番茄灰霉病防效为68.8%, 防治效果明显。

关键词：海洋生境芽孢杆菌T28菌株; 番茄; 多种功能

收稿：2015-05-08   修定：2015-10-30

资助：国家高技术“863”研究发展计划(2011AA10A202-2)、“十二五”国家科技支撑计划(2011BAE06B04)。

Multifunction of Marine Bacillus sp. Strain T28 on Tomato Growth-Promoting

CUI Rong-Qiang^1,2, ZHANG Jiu-Ming¹, MA Xiang-Jun¹, LI Chuang¹, TIAN Li^1,2,*

¹Biology Department, Qingdao University of Science & Technology, Qingdao, Shandong 266042, China; ²First Institute of Oceanography, State Oceanic Administration, Qingdao, Shandong 266061, China

Corresponding author: TIAN Li; E-mail: tianli@fio.org.cn; Tel: 0532-88967423

Abstract:

In this study, the multifunction on tomato such as nifH gene and nitrogen fixation, phosphate- and potassium-dissolving, siderophore and indole-3-acetic acid (IAA) production abilities, was investigated using marine Bacillus sp. strain T28 in vitro and in vivo. With gene nifH, proB, siderophore and IAA production inferred that the strain promoted tomato growth by nitrogen fixation, phosphate- and potassium-dissolving and proline gathering to resist salt stress. The chlorophyll content in tomato seedlings was increased 33.3% after marked strain colonization 15 d and control effects on Botrytis cinerea was 68.8%.

Key words: marine Bacillus sp. strain T28; tomato; multifunction